A strategy to ensure safety of stem cell-derived retinal pigment epithelium cells

Cell replacement and regenerative therapy using embryonic stem cell-derived material holds promise for the treatment of several pathologies. However, the safety of this approach is of prime importance given the teratogenic potential of residual stem cells, if present in the differentiated cell product. Using the example of embryonic stem cell-derived retinal pigment epithelium (RPE) for the treatment of age-related macular degeneration, we present a novel strategy for ensuring the absence of stem cells in the RPE population. Based on an unbiased screening approach, we identify and validate the expression of CD59, a cell surface marker expressed on RPE but absent on stem cells. We further demonstrate that flow sorting on the basis of CD59 expression can effectively purify RPE and deplete stem cells, resulting in a population free from stem cell impurity. This purification helps to ensure removal of stem cells and hence increases the safety of cells that may be used for clinical transplantation. This strategy can potentially be applied to other pluripotent stem cell-derived material and help mitigate concerns of using such cells for therapy

Metastable States and Leakage in PN Junction Diodes

The low-temperature annealing kinetics of ion implanted silicon is a critical factor worthy of consideration when thin film crystalline silicon is processed on a low temperature substrate. Deep Level Transient Spectroscopy, Thermally Stimulated Current and Current-Voltage characteristics suggest that competition between Solid Phase Epitaxial Regrowth and dislocation loop formation is a critical factor in silicon implanted with P31 ions and annealed at temperatures ranging from 525°C to 65O°C. Solid Phase Epitaxy dominates the kinetics of fully amorphized samples annealed at 525°C, where shallow-level trap states dominate the reversed-bias leakage current of diodes. At low implanted doses (where the substrate is not completely amorphized) and at 65O°C, dislocation loop nucleation and growth seems to dominate annealing kinetics, resulting in the formation of a deep-level trap at .528 eV

Theoretical Description of the Role of Halides, Silver, and Surfactants on the Structure of Gold Nanorods

Density functional theory simulations including dispersion provide an atomistic description of the role of different compounds in the synthesis of gold-nanorods. Anisotropy is caused by the formation of a complex between the surfactant, bromine, and silver that preferentially adsorbs on some facets of the seeds, blocking them from further growth. In turn, the nanorod structure is driven by the perferential adsorption of the surfactant, which induces the appearance of open {520} lateral facets

Universality of Load Balancing Schemes on Diffusion Scale

We consider a system of $N$ parallel queues with identical exponential service rates and a single dispatcher where tasks arrive as a Poisson process. When a task arrives, the dispatcher always assigns it to an idle server, if there is any, and to a server with the shortest queue among $d$ randomly selected servers otherwise $(1 \leq d \leq N)$. This load balancing scheme subsumes the so-called Join-the-Idle Queue (JIQ) policy $(d = 1)$ and the celebrated Join-the-Shortest Queue (JSQ) policy $(d = N)$ as two crucial special cases. We develop a stochastic coupling construction to obtain the diffusion limit of the queue process in the Halfin-Whitt heavy-traffic regime, and establish that it does not depend on the value of $d$, implying that assigning tasks to idle servers is sufficient for diffusion level optimality

A Cross-Cultural Analysis of Sex Differences in the Behavior of Children Aged Three Through 11

This paper uses the cross-cultural, systematic child observations of the Six Culture Study, led by John and Beatrice Whiting of Harvard University, to investigate the validity of the stereotypes of sex differences about nurturance, aggression, compliance, dependency, and other behaviors. The children aged 3 – 11 years, were observed in natural settings in seven different parts of the world. The analysis indicates that there are universal sex differences in the children’s behavior, but the differences are not consistent nor as great as the studies of American and Western European children would suggest. Furthermore, socialization pressure in the form of task assignment and the associated frequency of interaction with many different categories of individuals—i.e. infants, adults, and peers—may well explain many of the differences. Aggression, perhaps especially rough and tumble play, and touching behavior seem the best candidates for biophysical genesis. All of the behaviors that are characteristic of males and females seem malleable under the impact of socialization pressures. The differences in many of the types of behavior seem to be one of style rather than intent

Acceptance and commitment therapy delivered in a dyad after a severe traumatic brain injury: a feasibility study

Objective: There is a high prevalence of complex psychological distress after a traumatic brain injury but limited evidence of effective interventions. We examined the feasibility of Acceptance and Commitment Therapy after a severe traumatic brain injury using the criteria, investigating a therapeutic effect, and reviewing the acceptability of measures, treatment protocol, and delivery method (in a dyad of two clients and a therapist). Method: Two male outpatients with severe traumatic brain injury and associated psychological distress jointly engaged in a seven session treatment program based on Acceptance and Commitment Therapy principles. Pre- and post-treatment measures of mood, psychological flexibility, and participation were taken in addition to weekly measures. Results: The intervention showed a therapeutic effect with one participant, and appeared to be acceptable for both participants with regard to program content, measures, and delivery mode by in a dyad. One participant showed both significant clinical and reliable change across several outcome measures including measures of mood and psychological flexibility. The second participant did not show a reduction in psychological inflexibility, but did show a significant drop in negative affect. Significant changes pre- to post-treatment for measures of participation were not indicated. Qualitatively, both participants engaged in committed action set in accordance with their values. Conclusions: This study suggests that Acceptance and Commitment Therapy may be feasible to be delivered in a dyad with individuals who have a severe traumatic brain injury. A further test of its potential efficacy in a phase II clinical trial is recommended

The Optical Emission from Gamma-ray Quasars

We present photometric observations of six radio-loud quasars that were detected by the COMPTEL gamma-ray telescope. The data encompasses seven wavebands in the optical and near-infrared. After correction for Galactic extinction, we find a wide range in optical slopes. Two sources are as blue as optically-selected quasars, and are likely to be dominated by the accretion disc emission, while three others show colours consistent with a red synchrotron component. We discuss the properties of the COMPTEL sample of quasars, as well as the implications our observations have for multi-wavelength modelling of gamma-ray quasars.Comment: 12 pages, 3 figures, accepted for publication in P.A.S.A; minor typos correcte

Relationships between the El-Niño Southern Oscillation and spate flows in southern Africa and Australia

International audienceThe flow records of arid zone rivers are characterised by a high degree of seasonal variability, being dominated by long periods of very low or zero flow. Discrete flow events in these rivers are influenced by aseasonal factors such as global climate forcings. The atmospheric circulations of the El-Niño Southern Oscillation (ENSO) have been shown to influence climate regimes across many parts of the world. Strong teleconnections between changing ENSO regimes and discharges are likely to be observed in highly variable arid zones. In this paper, the influence of ENSO mechanisms on the flow records of two arid zone rivers in each of Australia and Southern Africa are identified. ENSO signals, together with multi-decadal variability in their impact as identified through seasonal values of the Interdecadal Pacific Oscillation (IPO) index, are shown to influence both the rate of occurrence and the size of discrete flow episodes in these rivers. Keywords: arid zones, streamflow, spates, climate variability, ENSO, Interdecadal Pacific Oscillation, IP